- Scientists at the CSIR-Centre for Cellular and Molecular Biology (CCMB) in Hyderabad have developed a new genetic toolkit to study the inner workings of Mycobacterium tuberculosis (M.tb). - The toolkit helps overcome challenges in understanding tuberculosis (TB) gene functions, particularly given the slow growth and complex cell wall of the TB pathogen. - This genetic innovation aims to accelerate target identification for drug discovery and better combat antimicrobial resistance (AMR) and drug-resistant TB strains. - **CSIR-Centre for Cellular and Molecular Biology (CCMB):** A premier research establishment under the Council of Scientific and Industrial Research (CSIR) in Hyderabad, focusing on frontier areas of modern biology, including cellular regulation, genetics, and infectious diseases. **Introduction** Researchers at the CSIR-Centre for Cellular and Molecular Biology (CCMB) have engineered a specialized genetic toolkit designed to investigate the inner biological mechanisms of Mycobacterium tuberculosis. Because TB bacteria are notoriously difficult to study due to their complex cell wall and slow replication, this toolkit provides a robust platform to analyze gene functions, identify vulnerable therapeutic targets, and support the development of novel interventions against drug-resistant tuberculosis. **Core Issue and Detailed Points** - Scientists at the CSIR-Centre for Cellular and Molecular Biology (CCMB) in Hyderabad have developed a new genetic toolkit to study the inner workings of Mycobacterium tuberculosis (M.tb). - The toolkit helps overcome challenges in understanding tuberculosis (TB) gene functions, particularly given the slow growth and complex cell wall of the TB pathogen. - This genetic innovation aims to accelerate target identification for drug discovery and better combat antimicrobial resistance (AMR) and drug-resistant TB strains. **Key Statutory Bodies, Acts & Concepts** - **CSIR-Centre for Cellular and Molecular Biology (CCMB):** A premier research establishment under the Council of Scientific and Industrial Research (CSIR) in Hyderabad, focusing on frontier areas of modern biology, including cellular regulation, genetics, and infectious diseases.